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Western blot analysis of ATF1 Antibody in Jurkat cell line lysates (35ug/lane)
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ATF1 Antibody in Jurkat cell line lysates (35ug/lane)

ATF1 Antibody

CATALOG NUMBER: 61-688

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This ATF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-219 amino acids from the C-terminal region of human ATF1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATF1
Additional Names:
Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1, Activating transcription factor 1, Protein TREB36, ATF1
Protein Accession Number:
P18846
Protein Gi Number:
1168542
Ncbi Gene Id Number:
466
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. ATF1 binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes.
Background Reference 1:
Wang, W.L., et al. Mod. Pathol. 22(9):1201-1209(2009)
Background Reference 2:
Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009)
Background Reference 3:
Zheng, D., et al. Cancer Res. 68(18):7650-7660(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-688

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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