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Western blot analysis of extracts from HT29 cells treated with Insulin using Phospho-ATF1 (Ser63) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from HT29 cells treated with Insulin using Phospho-ATF1 (Ser63) Antibody. The lane on the right is treated with the antigen-specific peptide.

ATF1 (phospho Ser63) Antibody

CATALOG NUMBER: 79-965

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
ATF1 (phospho Ser63) antibody was raised against a peptide sequence around phosphorylation site of serine 63 (R-P-S (p)-Y-R) derived from Human ATF1 .
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous level of ATF1 only when phosphorylated at serine 63.
Predicted Molecular Weight:
29 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
ATF1
Additional Names:
ATF1, Activating transcription factor 1, Cyclic-AMP-dependent transcription factor ATF-1, TCR-ATF1, TREB36 protein
Protein Accession Number:
NP_005162.1
Protein Gi Number:
4885073
Ncbi Gene Id Number:
466
Background:
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.
Background Reference 1:
Mackenzie KF, et al. (2013) J Immunol 190, 565-77
Background Reference 2:
Dickinson RJ, et al. (2011) J Biol Chem 286, 38018-26
Background Reference 3:
Wang J, Zhou JY, Wu GS (2011) J Biol Chem 286, 22384-92

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-965

List Size:
0.1 mg

List Price:

$355.00

Out of stock

Datasheet
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