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Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using ATF2 (Phospho-Thr71 or 53).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 (Phospho-Thr73 or 55).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using ATF2 (Phospho-Thr73 or 55).
Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using ATF2 (Phospho-Thr71 or 53).

ATF2 (phospho Thr73 / 55) Antibody

CATALOG NUMBER: 79-115

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ATF2 (Phospho-Thr73 or 55) antibody was raised against a peptide sequence around phosphorylation site of threonine 73 or 55 (T-P-T (p) -R-F) derived from Human ATF2.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of ATF-2 only whenphosphorylated at threonine 73 or 55.
Predicted Molecular Weight:
65 - 75 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
ATF2
Additional Names:
HB16, CREB2, TREB7, CREB-2, CRE-BP1, APRF, CREBP1
Protein Accession Number:
NP_001871.2
Protein Gi Number:
22538422
Ncbi Gene Id Number:
1386
Background:
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.
Background Reference 1:
Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101 (33): 12130-12135. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. Gupta S, et al. (1995) Science. 267: 389-393. Van Dam H, et al. (1995) EMBO J. 14 (8): 1798-1811. Livingstone C, et al. (1995) EMBO J. 14: 1785-1797.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-115

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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