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Western blot analysis in CEM cell line lysates (35ug/lane).
Western blot analysis of ATF3 using rabbit polyclonal ATF3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATF3 gene.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in CEM cell line lysates (35ug/lane).

ATF3 Antibody

CATALOG NUMBER: 58-581

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Bovine
Immunogen:
This ATF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-168 amino acids from the C-terminal region of human ATF3.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
21 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATF3
Additional Names:
Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3, Activating transcription factor 3, ATF3
Protein Accession Number:
P18847
Protein Gi Number:
1168543
Ncbi Gene Id Number:
467
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Activating transcription factor 3 is a member of themammalian activation transcription factor/cAMP responsiveelement-binding (CREB) protein family of transcription factors.Multiple transcript variants encoding two different isoforms havebeen found for this gene. The longer isoform represses rather thanactivates transcription from promoters with ATF binding elements.The shorter isoform (deltaZip2) lacks the leucine zipperprotein-dimerization motif and does not bind to DNA, and itstimulates transcription presumably by sequestering inhibitoryco-factors away from the promoter. It is possible that alternativesplicing of the ATF3 gene may be physiologically important in theregulation of target genes.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Lee, S.H., et al. Oncogene 29(37):5182-5192(2010)
Background Reference 3:
Park, H.J., et al. Biochem. Biophys. Res. Commun. 400(1):72-77(2010)
Background Reference 4:
Wu, X., et al. Nature 465(7296):368-372(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-581

List Size:
400 ul

List Price:

$495.00

Datasheet
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