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Western blot testing of human samples with ATF6 antibody (AF6229) at 2ug/ml.
IHC testing of FFPE human breast tissue with ATF6 antibody at 5ug/ml.
Western blot testing of human samples with ATF6 antibody (AF6229) at 2ug/ml.

ATF6 Antibody [AF6229]

CATALOG NUMBER: 33-034

Clonality:
Monoclonal
Tested Applications:
IHC, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A recombinant protein fragment from the N-terminal region of human ATF6 was used as the immunogen for this antibody.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western blot: 2-4 ug/ml (2)
IHC (Paraffin): 5 ug/ml (1)
ATF6 is a 670 amino acid protein that migrates in western blot between 70~90 kD due to glycosylation.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. ATF6 is seen in western blotting between 75~90KD.
Purification:
Protein G purified monoclonal antibody
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.5 mg/mL
Storage Conditions:
Aliquot and Store at -20°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
ATF6
Additional Names:
ATF6 Antibody: ATF6A, Cyclic AMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, cAMP-dependent transcription factor ATF-6 alpha
Ncbi Gene Id Number:
22926
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
ER stress can cause the accumulation of unfolded proteins and lead to the activation of the UPR (unfolded proetin response). Cells have developed an intracellular signaling pathway to deal with this stress, and Activating Transcription Factor 6 (ATF6) is a major transducer of the UPR. Upon activation, ATF6 seperates from GPR78 and is cleaved. The ~50 kD cytosolic portion of ATF6 translocates to the nucleus, transmitting the ER stress signal. In the nucleus, ATF6 activates the transcription of UPR-responsive genes by biniding to their ERSE (ER stress response element) region.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-034

List Size:
100 ug

List Price:

$520.00

Datasheet
Shipping Info

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