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46-814 (0.1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-814 (3.75ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-814 (0.1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

ATG16L1 Antibody

CATALOG NUMBER: 46-814

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KVEKVLSKQHSSSIN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 65kDa band observed in Mouse Brain lysates (calculated MW of 68.1kDa according to Mouse NP_084122.2). Recommended concentration: 0.01-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_110430.5 and NP_060444.3).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ATG16L1
Additional Names:
ATG16 , autophagy related 16-like 1 (S. cerevisiae) , hCG_1817841, APG16L, ATG16L, FLJ00045, FLJ10035, FLJ10828, FLJ22677, WDR30, APG16 autophagy 16-like, APG16L beta, ATG16 autophagy related 16-like protein 1, WD repeat domain 30
Protein Accession Number:
NP_110430.5,NP_060444.3
Protein Gi Number:
124256480
Ncbi Gene Id Number:
55054
Background Reference 1:
Rioux JD, Xavier RJ, Taylor KD, Silverberg MS, Goyette P, Huett A, Green T, Kuballa P, Barmada MM, Datta LW, Shugart YY, Griffiths AM, Targan SR, Ippoliti AF, Bernard EJ, Mei L, Nicolae DL, Regueiro M, Schumm LP, Steinhart AH, Rotter JI, Duerr RH, Cho JH, Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis. Nat Genet. 2007 May;39(5):596-604. Epub 2007 Apr 15.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-814

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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