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Western blot analysis of ATG2B in K562 cell lysate with ATG2B antibody at (A) 1 and (B) 2 μg/ml
Immunofluorescence of ATG2B in K562 cells with ATG2B antibody at 20 μg/mL.
Western blot analysis of ATG2B in K562 cell lysate with ATG2B antibody at (A) 1 and (B) 2 μg/ml
Trial Size

ATG2B Antibody

CATALOG NUMBER: 7005

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
ATG2B antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATG2B.
The immunogen is located within amino acids 450 - 500 of ATG2B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
ATG2B antibody can be used for detection of ATG2B by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
ATG2B antibody is predicted to not cross-react with other ATG2A.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 17-004 - K-562 Cell Slide
Predicted Molecular Weight:
229 kDa
Purification:
ATG2B Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ATG2B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ATG2B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATG2B
Additional Names:
ATG2B Antibody: C14orf103, C14orf103, Autophagy-related protein 2 homolog B
Protein Accession Number:
NP_060506
Protein Gi Number:
118197272
Ncbi Gene Id Number:
55102
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ATG2B Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is ATG2B, one of two homologs of ATG2 that is essential for autophagosome formation and important for regulation of size and distribution of lipid droplets. Relatively high rates of ATG2B mutations were observed in gastric and colorectal carcinomas, suggesting that deregulating the autophagy process may contribute to cancer development.
Background Reference 1:
Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
Background Reference 2:
Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
Background Reference 3:
Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
Background Reference 4:
Velikkakath AK, Nishimura T, Oita E, et al. Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets. Mol. Biol. Cell 2012; 23:896-909.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7005

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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