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Antibody used in WB on Human HepG2 at 1 ug/ml.
Antibody used in IHC on Human Placenta.
Antibody used in IHC on Human Prostate.
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.
Antibody used in WB on Human HepG2 at 1 ug/ml.

ATG5 Antibody

CATALOG NUMBER: 30-955

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATG5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ATG5 antibody can be used for detection of ATG5 by ELISA at 1:1562500. ATG5 antibody can be used for detection of ATG5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
32 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ATG5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ATG5
Additional Names:
ATG5, APG5, APG5L, ASP, hAPG5, APG5-LIKE
Protein Accession Number:
NP_004840
Protein Gi Number:
4757798
Ncbi Gene Id Number:
9474
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 may play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
Background Reference 1:
Ewing, R.M., Mol. Syst. Biol. 3, 89 (2007).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-955

List Size:
100 ul

List Price:

$519.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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