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45-306 (0.5ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-306 (2.5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-306 (0.5ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

ATGL Antibody

CATALOG NUMBER: 45-306

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-NIIEVSKEARKR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in Human Adipose lysates (calculated MW of 55.3kDa according to NP_065109.1). Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Testis for this particular batch.Immunohistochemistry:In paraffin embedded Human Heart shows intense staining of distinct spots in the myocardial fibres in transverse section. Recommended concentration: 2-3ug/ml.
Specificity:
Pease note this antibody was designed using the Mouse sequence NP_080078.1, which differs by 2 amino acids from the Human sequence NP_065109.1.
Positive Control 1:
Cat. No. XBL-11048 - Human Adipose Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PNPLA2
Additional Names:
ATGL, DESNUTRIN, PNPLA2, patatin-like phospholipase domain containing 2, 1110001C14Rik, DKFZp667M109, FP17548, PEDF-R, TTS-2.2, TTS2,adipose triglyceride lipase, pigment epithelium-derived factor, transport-secretion protein 2.2, triglyceride hydrolase
Protein Accession Number:
NP_065109.1
Protein Gi Number:
21313274
Ncbi Gene Id Number:
57104
Background Reference 1:
Schoenborn V, Heid IM, Vollmert C, Lingenhel A, Adams TD, Hopkins PN, Illig T, Zimmermann R, Zechner R, Hunt SC, Kronenberg F. The ATGL gene is associated with free fatty acids, triglycerides, and type 2 diabetes. Diabetes. 2006 May;55(5):1270-5.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-306

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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