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Western blot analysis of ATMIN in 293 cell lysate with ATMIN antibody at (A) 1 and (B) 2 μg/ml.
Immunocytochemistry of ATMIN in A431 cells with ATMIN antibody at 5 μg/mL.
Immunofluorescence of ATMIN in A431 cells with ATMIN antibody at 2.5 μg/mL.
Western blot analysis of ATMIN in 293 cell lysate with ATMIN antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

ATMIN Antibody

CATALOG NUMBER: 7777

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ATMIN antibody was raised against a 19 amino acid peptide near the amino terminus of human ATMIN.
The immunogen is located within amino acids 200 - 250 of ATMIN.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
ATMIN antibody can be used for detection of ATMIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
ATMIN antibody is human, mouse and rat reactive. At least three isoforms of ATMIN are known to exist.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-002 - A-431 Cell Slide
Predicted Molecular Weight:
Predicted: 73, 79, 91 kDa
Observed: 72 kDa
Purification:
ATMIN antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ATMIN Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ATMIN antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
ATMIN
Additional Names:
ATMIN Antibody: ASCIZ, ZNF822, KIAA0431, ATM interactor, Zinc finger protein 822, ASCIZ
Protein Accession Number:
NP_056066
Protein Gi Number:
54792092
Ncbi Gene Id Number:
23300
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The ATM/ATR-substrate CHK2-interacting zinc finger protein (ATMIN), also known as ASCIZ, forms DNA damage-induced nuclear foci that contain the DNA repair protein Rad51 (1). ATMIN is also thought to be involved in embryonic development, as an absence of ATMIN causes late-embryonic lethality in mice with a range of organ development defects (2). It also activates the transcription DYNLL1, a light chain of the dynein motor complex and sequence-specific regulator of protein dimerization of numerous targets. DYNLL1 can bind to and inhibit the transcription activation domain of ATMIN, forming a simple dynamic feedback loop for DYNLL1 expression (3).
Background Reference 1:
McNees CJ, Conlan LA, Tenis N, et al. ASCIZ regulates lesion-specific Rad51 focus formation and apoptosis after methylating DNA damage. EMBO J. 2005; 24:2447-57.
Background Reference 2:
Jurado S, Smyth I, van Denderen B, et al. Dual functions of ASCIZ in the DNA base damage response and pulmonary organogenesis. PLoS Genet. 2010; 6:e1001170.
Background Reference 3:
Jurado S, Conlan LA, Baker EK, et al. ATM substrate Chk2-interating Zn2+ finger (ASCIZ) is a bi-functional transcriptional activator and feedback sensor in the regulation of Dynein Light Chain (DYNLL1) expression. J. Biol. Chem. 2012; 287:3156-64.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7777

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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