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Western blot analysis of ATP12A Antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skin reacted with ATP12A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ATP12A Antibody in K562 cell line lysates (35ug/lane)

ATP12A Antibody

CATALOG NUMBER: 63-588

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ATP12A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 833-859 amino acids from the Central region of human ATP12A.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
116 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATP12A
Additional Names:
Potassium-transporting ATPase alpha chain 2, Non-gastric H(+)/K(+) ATPase subunit alpha, Proton pump, ATP12A, ATP1AL1
Protein Accession Number:
P54707
Protein Gi Number:
212287925
Ncbi Gene Id Number:
479
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ATP12A belongs to the family of P-type cation transport ATPases. This protein is a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues.
Background Reference 1:
Li,J., et.al., Kidney Int. 65 (4), 1301-1310 (2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-588

List Size:
400 ul

List Price:

$495.00

Datasheet
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