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Western blot analysis of lysates from K562 cell line and rat liver tissue lysate(from left to right), using ATP1B2 Antibody at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ATP1B2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysates from K562 cell line and rat liver tissue lysate(from left to right), using ATP1B2 Antibody at 1:1000 at each lane.

ATP1B2 Antibody

CATALOG NUMBER: 63-865

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rabbit, Rat
Immunogen:
This ATP1B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-141 amino acids from the Central region of human ATP1B2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATP1B2
Additional Names:
Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule in glia, AMOG, Sodium/potassium-dependent ATPase subunit beta-2, ATP1B2
Protein Accession Number:
P14415
Protein Gi Number:
125987795
Ncbi Gene Id Number:
482
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Background Reference 1:
Guey,L.T., et.al., Eur. Urol. 57 (2), 283-292 (2010)
Background Reference 2:
Tokhtaeva,E., et.al., Biochemistry 48 (48), 11421-11431 (2009)
Background Reference 3:
Hosgood,H.D. et.al., Respir Med 103 (12), 1866-1870 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-865

List Size:
400 ul

List Price:

$495.00

Datasheet
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