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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
ATP1B3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

ATP1B3 Antibody

CATALOG NUMBER: 56-621

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ATP1B3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-270 amino acids from the C-terminal region of human ATP1B3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
32 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATP1B3
Additional Names:
Sodium/potassium-transporting ATPase subunit beta-3, Sodium/potassium-dependent ATPase subunit beta-3, ATPB-3, CD298, ATP1B3
Protein Accession Number:
P54709
Protein Gi Number:
1703470
Ncbi Gene Id Number:
483
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the family ofNa+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamilyof Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane proteinresponsible for establishing and maintaining the electrochemicalgradients of Na and K ions across the plasma membrane. Thesegradients are essential for osmoregulation, for sodium-coupledtransport of a variety of organic and inorganic molecules, and forelectrical excitability of nerve and muscle. This enzyme iscomposed of two subunits, a large catalytic subunit (alpha) and asmaller glycoprotein subunit (beta). The beta subunit regulates,through assembly of alpha/beta heterodimers, the number of sodiumpumps transported to the plasma membrane. The glycoprotein subunitof Na+/K+ -ATPase is encoded by multiple genes. This gene encodes abeta 3 subunit. This gene encodes a beta 3 subunit. A pseudogeneexists for this gene, and it is located on chromosome 2. [providedby RefSeq].
Background Reference 1:
Floyd, R.V., et al. Reprod Sci 17(4):366-376(2010)
Background Reference 2:
Aughey, R.J., et al. J. Appl. Physiol. 103(1):39-47(2007)
Background Reference 3:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Background Reference 4:
Chiampanichayakul, S., et al. Tissue Antigens 68(6):509-517(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-621

List Size:
400 ul

List Price:

$495.00

Datasheet
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