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Western blot analysis of lysates from RD,HT1080 cell line (from left to right),using ATP2A1 Antibody .AP4905b was diluted at 1:1000 at each lane.
ATP2A1 Antibody IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysates from RD,HT1080 cell line (from left to right),using ATP2A1 Antibody .AP4905b was diluted at 1:1000 at each lane.

ATP2A1 Antibody

CATALOG NUMBER: 61-704

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rb
Immunogen:
This ATP2A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 974-1001 amino acids from the C-terminal region of human ATP2A1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
110 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATP2A1
Additional Names:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, SERCA1, SR Ca(2+)-ATPase 1, Calcium pump 1, Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, ATP2A1
Protein Accession Number:
O14983
Ncbi Gene Id Number:
487
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ATP2A1 encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction.
Background Reference 1:
Salanova, M., et al. Histochem. Cell Biol. 132(4):383-394(2009)
Background Reference 2:
Thorleifsson, G., et al. Nat. Genet. 41(1):18-24(2009)
Background Reference 3:
Chami, M., et al. Mol. Cell 32(5):641-651(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-704

List Size:
400 ul

List Price:

$495.00

Datasheet
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