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Antibody used in WB on Human MCF7 at 1 ug/ml.
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.
Antibody used in WB on Human MCF7 at 1 ug/ml.

ATP2A3 Antibody

CATALOG NUMBER: 26-148

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP2A3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ATP2A3 antibody can be used for detection of ATP2A3 by ELISA at 1:312500. ATP2A3 antibody can be used for detection of ATP2A3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
109 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ATP2A3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ATP2A3
Additional Names:
ATP2A3, SERCA3
Protein Accession Number:
NP_005164
Protein Gi Number:
28373103
Ncbi Gene Id Number:
489
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ATP2A3 is one of the SERCA Ca (2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction.This gene encodes one of the SERCA Ca (2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms.
Background Reference 1:
Korosec, B., (2008) Cancer Genet. Cytogenet. 181 (2), 112-118.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-148

List Size:
100 ul

List Price:

$519.00

Datasheet
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