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Western blot analysis of ATP4B Antibody in mouse heart tissue lysates (35ug/lane)
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ATP4B Antibody in mouse heart tissue lysates (35ug/lane)

ATP4B Antibody

CATALOG NUMBER: 61-824

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This ATP4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-78 amino acids from the N-terminal region of human ATP4B.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATP4B
Additional Names:
Potassium-transporting ATPase subunit beta, Gastric H(+)/K(+) ATPase subunit beta, Proton pump beta chain, ATP4B
Protein Accession Number:
P51164
Protein Gi Number:
1703461
Ncbi Gene Id Number:
496
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ATP4B belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.
Background Reference 1:
Bab-Dinitz, E., et al. Biochemistry 48(36):8684-8691(2009) Knouff, C.W., et al. Pharmacogenet. Genomics 18(12):1051-1057(2008) Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-824

List Size:
400 ul

List Price:

$495.00

Datasheet
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