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Western blot analysis in WiDr,NCI-H460,MDA-MB231 cell line lysates (35ug/lane).
ATP5A1 Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in WiDr,NCI-H460,MDA-MB231 cell line lysates (35ug/lane).

ATP5A1 Antibody

CATALOG NUMBER: 64-109

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat
Immunogen:
This ATP5A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 477-503 amino acids from the C-terminal region of human ATP5A1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
60 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATP5A1
Additional Names:
ATP synthase subunit alpha, mitochondrial, ATP5A1, ATP5A, ATP5AL2, ATPM
Protein Accession Number:
P25705
Protein Gi Number:
114517
Ncbi Gene Id Number:
498
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core.
Background Reference 1:
Pandey, N.R., et al. Am. J. Pathol. 175(4):1777-1787(2009)
Background Reference 2:
Seth, R., et al. J. Clin. Pathol. 62(7):598-603(2009)
Background Reference 3:
Law, I.K., et al. Proteomics 9(9):2444-2456(2009)
Background Reference 4:
Martins-de-Souza, D., et al. BMC Psychiatry 9, 17 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-109

List Size:
400 ul

List Price:

$495.00

Datasheet
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