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43-539 (0.3ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-539 (0.3ug/ml) staining of Pig Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-539 (0.3ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ATP5B Antibody

CATALOG NUMBER: 43-539

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-EPIDERGPIKTKQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 55kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 56.6kDa according to NP_001677.2). Recommended concentration: 0.3-1ug/ml.
Predicted Molecular Weight:
Approx 55kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ATP5B
Additional Names:
ATP5B, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide, ATPMB, ATPSB, ATP synthase subunit beta, mitochondrial, mitochondrial ATP synthase beta subunit, mitochondrial ATP synthetase, beta subunit
Protein Accession Number:
NP_001677.2
Protein Gi Number:
32189394
Ncbi Gene Id Number:
506
Background Reference 1:
Yavlovich A, Viard M, Zhou M, Veenstra TD, Wang JM, Gong W, Heldman E, Blumenthal R, Raviv Y.Ectopic ATP synthase facilitates transfer of HIV-1 from antigen-presenting cells to CD4(+) target cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-539

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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