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43-547 (0.03ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-547 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-547 (0.03ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

ATP5F1 Antibody

CATALOG NUMBER: 43-547

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-SIQHIQNAIDTE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 25kDa band observed in Human Heart lysates (calculated MW of 28.9kDa according to NP_001679.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.
Predicted Molecular Weight:
Approx 25kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ATP5F1
Additional Names:
ATP5F1, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1, PIG47, ATP synthase B chain, mitochondrial, ATP synthase subunit b, mitochondrial, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1, ATP synthase, H+ transport
Protein Accession Number:
NP_001679.2
Protein Gi Number:
21361565
Ncbi Gene Id Number:
515
Background Reference 1:
Elston T, Wang H, Oster G.Energy transduction in ATP synthase.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-547

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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