Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of lysate from rat heart tissue lysate, using ATP5J Antibody at 1:1000.
Fluorescent confocal image of U251 cell stained with ATP5J Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATP5J primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ATP5J immunoreactivity is localized to Mitochondria significantly.
Western blot analysis in mouse stomach tissue lysates (35ug/lane).
ATP5J Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of lysate from rat heart tissue lysate, using ATP5J Antibody at 1:1000.

ATP5J Antibody

CATALOG NUMBER: 56-437

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This ATP5J antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-56 amino acids from the Central region of human ATP5J.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
13 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATP5J
Additional Names:
ATP synthase-coupling factor 6, mitochondrial, ATPase subunit F6, ATP5J, ATP5A, ATPM
Protein Accession Number:
P18859
Protein Gi Number:
114688
Ncbi Gene Id Number:
522
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Mitochondrial ATP synthase catalyzes ATP synthesis,utilizing an electrochemical gradient of protons across the innermembrane during oxidative phosphorylation. It is composed of twolinked multi-subunit complexes: the soluble catalytic core, F1, andthe membrane-spanning component, Fo, which comprises the protonchannel. The F1 complex consists of 5 different subunits (alpha,beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3beta, and a single representative of the other 3. The Fo seems tohave nine subunits (a, b, c, d, e, f, g, F6 and 8). This geneencodes the F6 subunit of the Fo complex, required for F1 and Fointeractions. Alternatively spliced transcript variants encodingdifferent isoforms have been identified for this gene. A pseudogeneexists on chromosome Yp11.
Background Reference 1:
Osanai, T., et al. Cardiovasc. Res. 81(4):780-787(2009)
Background Reference 2:
Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008)
Background Reference 3:
Kumagai, A., et al. Atherosclerosis 200(1):45-50(2008)
Background Reference 4:
Chai, S.B., et al. Circ. J. 71(5):693-697(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-437

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369