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Western blot analysis of extracts of various cell lines, using ATP6AP2 antibody (22-290) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using ATP6AP2 antibody (22-290). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ATP6AP2 antibody (22-290) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

ATP6AP2 Antibody

CATALOG NUMBER: 22-290

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 251-350 of human ATP6AP2 (NP_005756.2).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
Positive Control 1:
SW480
Positive Control 2:
Mouse kidney
Positive Control 3:
Mouse brain
Positive Control 4:
Mouse eye
Positive Control 5:
Rat brain
Positive Control 6:
Rat kidney
Predicted Molecular Weight:
Observed: 48kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ATP6AP2
Additional Names:
ATP6IP2, ATPase, H+ transporting, lysosomal interacting protein 2, M8-9, APT6M8-9, ATP6M8-9, renin receptor, V-ATPase M8.9 subunit, ATPase membrane sector associated protein M8-9, vacuolar ATP synthase membrane sector associated protein M8-9, lysosomal (vacuolar proton pump) membrane sector associated protein M8-9, ATP6IP2, CAPER, ELDF10, HT028, MSTP009, PSEC0072
Ncbi Gene Id Number:
10159
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-290

List Size:
100 uL

List Price:

$436.00

Datasheet
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