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Western blot analysis of various lysates using ATPB Mouse mAb at 1:10000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using ATPB Mouse mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ATPB Mouse mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using ATPB Mouse mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ATPB Mouse mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using ATPB Mouse mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of PC-12 cells using ATPB Mouse mAb followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of Hep G2 cells using ATPB Mouse mAb followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of HeLa cells using ATPB Mouse mAb followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of paraffin-embedded Mouse kidney tissue using ATPB Mouse mAb followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of NIH/3T3 cells using ATPB Mouse mAb followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Western blot analysis of various lysates using ATPB Mouse mAb at 1:10000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.

ATPB Mouse Recombinant Antibody (83-350)

CATALOG NUMBER: 83-350

Clonality:
Monoclonal
Tested Applications:
ELISA, IF-P, IF/ICC, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF-P, IF/ICC, IHC-P, WB
Application Note:
WB,1:10000 - 1:40000, IF/ICC,1:200 - 1:800, IF-P,1:200 - 1:800, IHC-P,1:2000 - 1:20000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
57kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ATP5F1B
Additional Names:
ATP5B; ATPMB; ATPSB; HUMOP2; HEL-S-271
Protein Accession Number:
P06576
Ncbi Gene Id Number:
506
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-350

List Size:
100 uL

List Price:

$434.00

Datasheet
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