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Western blot analysis using AURKB monoclonal antibody against truncated AURKB recombinant protein and SKN - SH cell lysate.
Western blot analysis using AURKB monoclonal antibody against truncated AURKB recombinant protein and SKN - SH cell lysate.

AURKB Antibody [13E8D3]

CATALOG NUMBER: 32-114

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant AURKB expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
Isotype: IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
AURKB monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AURKB
Additional Names:
AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5, Aurora 1, AIM-1, AIK2, AurB, IPL1, PPP1R48, aurkb-sv1, aurkb-sv2
Protein Accession Number:
Q96GD4
Protein Gi Number:
317373473
Ncbi Gene Id Number:
9212
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AURKB (aurora kinase B, AIK2 or aurora-B), with 344-amino acid protein(about 39 kDa),localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. AURKB is a mitotic protein kinase, which phosphorylates histone H3 and regulates Chromosomal segregation during mitosis and meiosis.It may regulates several stages of mitosis such as centrosome separation, chromosome segregation and cytokinesis.Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. ARK-2 transcripts arepresent at high levels in human thymus and fetal liver.ARK-2 protein levels are maximal during both S and G2/M phases
Background Reference 1:
Song J et al. Immunity 22:621-31 (2005).
Background Reference 2:
Kapoor P et al. Mol Cell Biol 25:4934-45 (2005).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-114

List Size:
0.1 mL

List Price:

$495.00

Datasheet
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