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Western blot testing of 1) a partial recombinant protein and 2) human liver lysate with Aurora B antibody (clone AURKB/1521). Predicted molecular weight: 39-45 kDa
SDS-PAGE Analysis of Purified, BSA-Free Aurora B Antibody (clone AURKB/1521). Confirmation of Integrity and Purity of the Antibody.
Western blot testing of 1) a partial recombinant protein and 2) human liver lysate with Aurora B antibody (clone AURKB/1521). Predicted molecular weight: 39-45 kDa

Aurora B Antibody [AURKB/1521]

CATALOG NUMBER: 33-614

Clonality:
Monoclonal
Tested Applications:
Flow, IF, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Amino acids 89-251 were used as the immunogen for this Aurora B antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
Western blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1ug/10e6 cells in 0.1ml
IF: 1-2 ug/ml
Optimal dilution of the Aurora B antibody should be determined by the researcher.
Purification:
Protein G affinity
Clonality:
Monoclonal
Isotype:
IgG2b
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
AURKB
Additional Names:
Aurora kinase B, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, ARK-2, Aurora-related kinase 2, STK-1, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 5, Serine/threonine-protein kinase aurora-B, AURKB, AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5
Ncbi Gene Id Number:
9212
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Recognizes a protein of 39-45kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-614

List Size:
100 ug

List Price:

$626.00

Datasheet
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