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The purity of rh ENPP2 / Autotaxin was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
The purity of rh ENPP2 / Autotaxin was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.

Autotaxin Recombinant Protein

CATALOG NUMBER: 96-264

Tested Applications:
WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Asp 49 - Ile 863
Fusion Tag:
His Tag
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
94.5 kDa
Purity:
>92% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
50 mM Tris, 150 mM NaCl, pH 7.5
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
ENPP2
Additional Names:
ENPP2, E-NPP 2, Autotaxin, LysoPLD, ATX, PDNP2, ATX-X, NPP2, PD-IALPHA
Protein Accession Number:
NP_001035181
Ncbi Gene Id Number:
5168
Background:
Autotaxin also known as ectonucleotide pyrophosphatase / phosphodiesterase family member 2 (E-NPP 2 or ENPP2), Extracellular lysophospholipase D (LysoPLD), Autotaxin (ATX or ATX-X), which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP2 contains two SMB (somatomedin-B) domains. ENPP2 hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. ENPP2 can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. ENPP2 stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein.
Background Reference 1:
Stracke M.L., et al., 1992, J. Biol. Chem. 267:2524-2529.
Background Reference 2:
Nam S.W., et al., 2001, Cancer Res. 61:6938-6944.
Background Reference 3:
Hausmann J., et al., 2011, Nat. Struct. Mol. Biol. 18:198-204.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-264

List Size:
0.05 mg

List Price:

$515.00

Datasheet
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