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Western blot analysis of AVIL Antibody in K562 cell line lysates (35ug/lane)
AVIL Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of AVIL Antibody in K562 cell line lysates (35ug/lane)

AVIL Antibody

CATALOG NUMBER: 61-651

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This AVIL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-204 amino acids from the N-terminal region of human AVIL.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
92 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AVIL
Additional Names:
Advillin, p92, AVIL
Protein Accession Number:
O75366
Ncbi Gene Id Number:
10677
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AVIL is a member of the gelsolin/villin family of actin regulatory proteins. This protein has structural similarity to villin. It binds actin and may play a role in the development of neuronal cells that form ganglia.
Background Reference 1:
Piana, S., et al. J. Mol. Biol. 375(2):460-470(2008)
Background Reference 2:
Vermeulen, W., et al. Protein Sci. 13(5):1276-1287(2004)
Background Reference 3:
Tumer, Z., et al. Gene 288 (1-2), 179-185 (2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-651

List Size:
400 ul

List Price:

$495.00

Datasheet
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