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Western blot analysis of AXIN1 in SK-N-SH cell lysate with AXIN1 antibody at 1 μg/mL.
Immunofluorescence of AXIN1 in human brain tissue with AXIN1 antibody at 20 μg/mL.
Western blot analysis of AXIN1 in SK-N-SH cell lysate with AXIN1 antibody at 1 μg/mL.
Trial Size

AXIN1 Antibody

CATALOG NUMBER: 6005

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (79%)
Immunogen:
AXIN1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human AXIN1.
The immunogen is located within amino acids 620 - 670 of AXIN1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
AXIN1 antibody can be used for detection of AXIN1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
AXIN1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
AXIN1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
AXIN1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AXIN1
Additional Names:
AXIN1 Antibody: AXIN, PPP1R49, AXIN, Axin-1, Axis inhibition protein 1, hAxin
Protein Accession Number:
AAC51624
Protein Gi Number:
2252820
Ncbi Gene Id Number:
8312
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AXIN1 Antibody: AXIN1 is a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain and is thought to function as a negative regulator of the WNT signaling pathway that regulates embryonic axis formation. AXIN1 interacts with adenomatosis polyposis coli (APC), beta-catenin, glycogen synthase kinase 3 beta, forming a tetrameric complex resulting in the regulation of the stabilization of beta-catenin. Mutations in the AXIN1 gene have been associated various carcinomas, indicating that it also functions as a tumor suppressor.
Background Reference 1:
Zeng L, Fagotto F, Zhang T, et al. The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation. Cell1997; 90:181-92.
Background Reference 2:
Kishida S, Yamamoto H, Ikeda S, et al. Axin, a negative regulator of the wnt signaling pathway, directly interacts with adenomatous polyposis coli and regulates the stabilization of beta-catenin. J. Biol. Chem.1998; 273:10823-6.
Background Reference 3:
Nakamura T, Hamada F, Ishidate T, et al. Axin, an inhibitor of the Wnt signaling pathway, interacts with beta-catenin, GSK-3beta and APC and reduces the beta-catenin level. Genes Cells1998; 3:395-403.
Background Reference 4:
Salahshor S and Woodgett JR. The links between axin and carcinogenesis. J. Clin. Pathol.2005; 58:225-36.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6005

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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