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Western blot analysis of anti-AXL N-term Pab in SKBR3 cell lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Flow cytometric analysis of WiDr cells using AXL Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of anti-AXL N-term Pab in SKBR3 cell lysate

AXL Antibody

CATALOG NUMBER: 63-029

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This AXL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-44 amino acids from the N-terminal region of human AXL.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
98 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AXL
Additional Names:
Tyrosine-protein kinase receptor UFO, AXL oncogene, AXL, UFO
Protein Accession Number:
P30530
Protein Gi Number:
239938818
Ncbi Gene Id Number:
558
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AXL, a member of the AXL/UFO subfamily of Tyr protein kinases, may function as a signal transducer between specific cell types of mesodermal origin. This Type I membrane protein has transforming potential in patients with chronic myeloproliferative disorder or chronic myelocytic leukemia. The protein contains 2 putative fibronectin type III domains and 2 putative immunoglobulin-like C2-type domains.
Background Reference 1:
Lee, S.T., et al., Oncogene 8(12):3403-3410 (1993).
Background Reference 2:
Janssen, J.W., et al., Oncogene 6(11):2113-2120 (1991).
Background Reference 3:
O'Bryan, J.P., et al., Mol. Cell. Biol. 11(10):5016-5031 (1991).
Background Reference 4:
Partanen, J., et al., Proc. Natl. Acad. Sci. U.S.A. 87(22):8913-8917 (1990).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-029

List Size:
400 ul

List Price:

$495.00

Datasheet
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