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To detect Human -beta-NGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human -beta-NGF (XP-5110Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human -beta-NGF.
To detect Human -beta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human -beta-NGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human -beta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human -beta-NGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 0.5 mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results were achieved without antigen retrieval. Optimal concentrations and conditions may vary.
To detect Human -beta-NGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human -beta-NGF (XP-5110Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human -beta-NGF.

B-NGF Antibody

CATALOG NUMBER: XP-5110

Clonality:
Polyclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hβ-NGF (Human beta-Nerve Growth Factor).
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hβ-NGF (1 ng/mL), a concentration of 0.05 - 0.08 μg/mL of this antibody is required.
ELISA:
To detect hβ-NGF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hβ-NGF.
Western Blot:
To detect hβ-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ-NGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hβ-NGF specific antibody was purified by affinity chromatography employing immobilized hβ-NGF matrix.
Clonality:
Polyclonal
Physical State:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
B-NGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
NGF
Additional Names:
NGFB, HSAN5, Beta-NGF, NGFB, Beta-nerve growth factorBeta-NGF
Protein Accession Number:
P01138
Protein Gi Number:
90110037
Ncbi Gene Id Number:
4803
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5110

List Size:
0.1 mg

List Price:

$385.00

Out of stock

Datasheet
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