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Western blot analysis of extracts from K562 cells treated with EGF using B-Raf (Phospho-Thr753) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from K562 cells treated with EGF using B-Raf (Phospho-Thr753) Antibody. The lane on the right is treated with the antigen-specific peptide.

B-Raf (phospho Thr753) Antibody

CATALOG NUMBER: 79-896

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
B-Raf (phospho Thr753) antibody was raised against a peptide sequence around phosphorylation site of threonine 753 (P-K-T (p)-P-L) derived from Human B-Raf.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 753
Predicted Molecular Weight:
84 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
BRAF
Additional Names:
B-RAF, RAFB1, RMIL, p94
Protein Accession Number:
NP_004324.2
Protein Gi Number:
50403720
Ncbi Gene Id Number:
673
Background:
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
Background Reference 1:
Stephens R.M., Mol. Cell. Biol. 12:3733-3742(1992).
Background Reference 2:
Albert S., Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Background Reference 3:
Scherer S.W., Science 300:767-772(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-896

List Size:
0.1 mg

List Price:

$376.00

Datasheet
Shipping Info

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