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Western blot analysis of B2M in SK-N-SH cell lysate with B2M antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of B2M in mouse brain tissue with B2M antibody 5 μg/mL.
Immunofluorescence of B2M in mouse brain tissue with B2M antibody at 20 μg/mL.
Western blot analysis of B2M in SK-N-SH cell lysate with B2M antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

B2M Antibody

CATALOG NUMBER: 7439

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (83%), Rat: (83%)
Immunogen:
B2M antibody was raised against a 15 amino acid peptide near the carboxy terminus of human B2M.
The immunogen is located within the last 50 amino acids of B2M.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
B2M antibody can be used for detection of B2M by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
B2M antibody is human reactive.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Predicted Molecular Weight:
Predicted: 13 kDa
Observed: 12 kDa
Purification:
B2M Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
B2M Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
B2M antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
B2M
Additional Names:
B2M Antibody: Beta-2-microglobulin
Protein Accession Number:
NP_004039
Protein Gi Number:
4757826
Ncbi Gene Id Number:
567
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
B2M Antibody: Beta2-microglobulin (B2M) is a principal component of the Major Histocompatibility Complex (MHC) class I molecule, a ternary membrane protein complex that displays fragments derived from proteolyzed cytosolic proteins on the surface of cells for recognition by the surveillance immune system (1,2). B2M is involved in the presentation of peptide antigens to the immune system and plays a critically important role in immune system function (3). It is expressed on nearly all nucleated cells and contains one Ig-like C1-type (immunoglobulin-like) domain (2,3). Mutations in the Beta 2-microglobulin gene can enhance the progression of malignant melanoma and osteoarthropathy (4,5).
Background Reference 1:
Krangel MS, Orr HT, and Strominger JL. Assembly and maturation of HLA-A and HLA-B antigens in vivo. Cell 1979; 18:979-91.
Background Reference 2:
Skjodt K, Welinder KG, Crone M, et al. Isolation and characterization of chicken and turkey beta 2-microglobulin. Mol. Immunol. 1986; 23:1301-9.
Background Reference 3:
Ohashi K. Pathogenesis of beta2-microglobulin amyloidosis. Pathol. Int. 2001; 51:1-10.
Background Reference 4:
Blum C, Graham A, Yousefzadeh M, et al. The expression ratio of Map7/B2M is prognostic for survival in patients with stage II colon cancer. Int. J. Oncol. 2008; 33:579-84.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7439

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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