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Western blot analysis of B3GALT6 Antibody in Ramos cell line lysates (35ug/lane)
B3GALT6 Antibody in Ramos cell line lysates (35ug/lane).B3GALT6 (arrow) ) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of B3GALT6 Antibody in Ramos cell line lysates (35ug/lane)

B3GALT6 Antibody

CATALOG NUMBER: 61-753

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This B3GALT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-269 amino acids from the C-terminal region of human B3GALT6.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
B3GALT6
Additional Names:
Beta-1,3-galactosyltransferase 6, Beta-1,3-GalTase 6, Beta3Gal-T6, Beta3GalT6, GAG GalTII, Galactosyltransferase II, Galactosylxylosylprotein 3-beta-galactosyltransferase, UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 6, B3GALT6
Protein Accession Number:
Q96L58
Protein Gi Number:
61211870
Ncbi Gene Id Number:
126792
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
B3GALT6, Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. B3GALT6 has a preference for galactose-beta-1,4-xylose that is found in the linker region of glycosaminoglycans, such as heparan sulfate and chondroitin sulfate. B3GALT6 has no activity towards substrates with terminal glucosamine or galactosamine residues.
Background Reference 1:
Bai, X., et al. J. Biol. Chem. 276(51):48189-48195(2001) Zhou, D., et al. Proc. Natl. Acad. Sci. U.S.A. 96(2):406-411(1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-753

List Size:
400 ul

List Price:

$495.00

Datasheet
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