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Western blot analysis of extracts of various cell lines, using B3GALTL antibody (23-774) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
Western blot analysis of extracts of various cell lines, using B3GALTL antibody (23-774) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.

B3GALTL Antibody

CATALOG NUMBER: 23-774

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 259-498 of human B3GALTL (NP_919299.3).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
U-251MG
Positive Control 2:
HeLa
Positive Control 3:
SKOV3
Positive Control 4:
HepG2
Positive Control 5:
MCF7
Predicted Molecular Weight:
Observed: 56kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
B3GALTL
Additional Names:
B3GALTL, B3GTL, B3Glc-T, Gal-T, beta3Glc-T, beta-1,3-glucosyltransferase, UDP-GAL:beta-GlcNAc beta-1,3-galactosyltransferase-like, beta 1,3-galactosyltransferase-like, beta 3-glycosyltransferase-like
Ncbi Gene Id Number:
145173
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a beta-1,3-glucosyltransferase that transfers glucose to O-linked fucosylglycans on thrombospondin type-1 repeats (TSRs) of several proteins. The encoded protein is a type II membrane protein. Defects in this gene are a cause of Peters-plus syndrome (PPS).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-774

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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