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Western blot analysis of B3GAT1 Antibody in K562 cell line lysates (35ug/lane)
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of B3GAT1 Antibody in K562 cell line lysates (35ug/lane)

B3GAT1 Antibody

CATALOG NUMBER: 64-189

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This B3GAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-48 amino acids from the N-terminal region of human B3GAT1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
38 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
B3GAT1
Additional Names:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Beta-1,3-glucuronyltransferase 1, Glucuronosyltransferase P, GlcAT-P, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase, GlcUAT-P, B3GAT1, GLCATP
Protein Accession Number:
Q9P2W7
Protein Gi Number:
205830910
Ncbi Gene Id Number:
27087
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7).
Background Reference 1:
Petrovas, C., et al. J. Immunol. 183(2):1120-1132(2009)
Background Reference 2:
Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009)
Background Reference 3:
Chong, L.K., et al. Eur. J. Immunol. 38(4):995-1000(2008)
Background Reference 4:
Casado, J.G., et al. Tumour Biol. 29(5):304-310(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-189

List Size:
400 ul

List Price:

$495.00

Datasheet
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