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<b> Binding curve of anti-B7-H3 antibody MJ18 (24-552) to mouse B7-H3-Fc fusion protein </b> ELISA Plate coated with mouse B7-H3-Fc fusion protein (Pr00158-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 to 0.1 ng/ml was performed using 24-552. For detection- a 1:4000 dilution of HRP-loat anti-ribody (Bio-Rad) was used.
<b> Immunofluorescence staining of fixed mouse splenocytes with anti-B7-H3 (CD276) antibody MJ18 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse (<i>Mus musculus</i>) splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric rsion of MJ18 (24-552) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing weak membrane staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-552- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody
Binding curve of anti-B7-H3 antibody MJ18 (24-552) to mouse B7-H3-Fc fusion protein ELISA Plate coated with mouse B7-H3-Fc fusion protein (Pr00158-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 to 0.1 ng/ml was performed using 24-552. For detection- a 1:4000 dilution of HRP-loat anti-ribody (Bio-Rad) was used.

B7-H3 Antibody [MJ18], Rabbit IgG

CATALOG NUMBER: 24-552

Clonality:
Recombinant Monoclonal
Tested Applications:
Blocking, Flow
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Mouse
Species:
Rabbit
Original Species Name:
Rat
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
This antibody was generated by immunizing Sprague Dawley rats with B7-H3-Ig, consisting of the extracellular domain (aa1–242 of mouse B7-H3) linked to the Fc portion of mouse IgG2a, emulsified in CFA (Difco). The antibody was selected by its reactivity to mouse B7-H3-transfected cells, but not to parental cells by flow cytometry.
Conjugate:
Unconjugated
Tested Applications:
Blocking, Flow
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
Cd276
Additional Names:
CD276, B7H3
Ncbi Gene Id Number:
102657
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-552

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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