B7-H5 Recombinant Protein

CATALOG NUMBER: 96-826

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Phe 33 - Ala 194
Fusion Tag:
Fc Tag
Tested Applications:
ELISA, WB
Application Note:
This recombinant protein can be used for E, WB. For research use only.
Predicted Molecular Weight:
44.8 kDa
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human VSIG8, His Tag at 10 ug/mL (100 uL/well) can bind Human B7-H5, Fc Tag with a linear range of 0.625-20 ug/mL.
Purity:
>95% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
B7-H5
Additional Names:
B7-H5,SISP1,Gi24,VISTA
Protein Accession Number:
AAH20568
Ncbi Gene Id Number:
64115
Background:
Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.
Background Reference 1:
Sakr M.A., et al., 2010, Cancer Sci. 101:2368-2374.
Background Reference 2:
Mayya V., et al ., 2009, Sci. Signal. 2:RA46-RA46.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-826

List Size:
0.1 mg

List Price:

$463.00

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