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<b> Flow cytometry using the Anti-B7-H6 antibody 17B1.3 ( </b> Paraformaldehyde fixed HeLa cells were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of 17B1.3 (24-776- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
<b> Immunofluorescence staining of fixed K562 cells with anti-B7-H6 antibody 17B1.3 (</b> Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips stained with the chimeric r version of 17B1.3 (24-776) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-776- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using the Anti-B7-H6 antibody 17B1.3 ( Paraformaldehyde fixed HeLa cells were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of 17B1.3 (24-776- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

B7-H6 Antibody [17B1.3], Rabbit IgG

CATALOG NUMBER: 24-776

Clonality:
Recombinant Monoclonal
Tested Applications:
Crystallography, Flow, IF
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human
Species:
Rabbit
Original Species Name:
Mouse
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
Immunised mice with extracellular domain of human B7-H6 and selected on the basis of specific reactivity by P815.B7-H6, but not with P815.B7-H1.
Conjugate:
Unconjugated
Tested Applications:
Crystallography, Flow, IF
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
NCR3LG1
Additional Names:
B7H6, B7 homolog 6, Natural cytotoxicity triggering receptor 3 ligand 1, NCR3LG1, DKFZp686O2416
Ncbi Gene Id Number:
374383
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-776

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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