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Western blot analysis of B9D1 in 293 cell lysate with B9D1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of B9D1 in 293 cells with B9D1 antibody at 5 μg/mL.
Immunofluorescence of B9D1 in 293 cells with B9D1 antibody at 20 μg/mL.
Western blot analysis of B9D1 in 293 cell lysate with B9D1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

B9D1 Antibody

CATALOG NUMBER: 6715

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%)
Immunogen:
B9D1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human B9D1.
The immunogen is located within amino acids 130 - 180 of B9D1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
B9D1 antibody can be used for detection of B9D1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of B9D1 are known to exist; this antibody will only recognize the longest isoform. B9D1 antibody is predicted to not cross-react with other DNAJC family members.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Purification:
B9D1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
B9D1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
B9D1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
B9D1
Additional Names:
B9D1 Antibody: B9, MKS9, EPPB9, MKSR1, B9 domain-containing protein 1, MKS1-related protein 1
Protein Accession Number:
NP_056496
Protein Gi Number:
343478275
Ncbi Gene Id Number:
27077
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
B9D1 Antibody: Meckel syndrome (MKS) is an embryonic lethal, autosomal recessive disorder characterized by polycystic kidney disease, central nervous system defects, polydactyly and liver fibrosis. B9D1 is a B9 domain-containing protein, one of several that are involved in ciliogenesis. Alterations in expression of this gene have been found in a family with Meckel syndrome. B9D1, and its related protein B9D2, form a complex with MKS1, disruption of which causes MKS. B9D1 is thought to be required for normal hedgehog signaling, ciliogenesis, and ciliary protein localization.
Background Reference 1:
Williams CL, Winkelbauer ME, Schafer JC, et al. Functional redundancy of the B9 proteins and nephocystins in Caenorhabditis elegans ciliogenesis. Mol. Biol. Cell 2008; 19:2154-68.
Background Reference 2:
Hopp K, Heyer CM, Hommerding CJ, et al. B9D1 is revealed as a novel Meckel syndrome (MKS) gene by targeted exon-enriched next-generation sequencing and deletion analysis. Hum. Mol. Genet. 2011; 20:2524-34.
Background Reference 3:
Dowdle WE, Robinson JF, Kneist A, et al. Disruption of a ciliary B9 protein complex causes Meckel syndrome. Am. J. Hum. Genet. 2011; 89:94-110.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6715

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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