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Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using BACE1 mAb against human BACE1 (AA: 112-324) recombinant protein. (Expected MW is 49.9 kDa)
Western blot analysis using BACE1 mAb against HEK293 (1) and BACE1 (AA: 112-324)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.
Flow cytometric analysis of Hela cells using BACE1 mouse mAb (green) and negative control (red).
Immunofluorescence analysis of MCF-7 cells using BACE1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

BACE1 Antibody [3C1C3]

CATALOG NUMBER: 32-238

Clonality:
Monoclonal
Tested Applications:
ELISA, Flow, ICC, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rurified recombinant fragment of human BACE1 (AA: 112-324) expressed in E. Coli.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, ICC, WB
Application Note:
ELISA: 1/10000
Western Blot: 1/500 - 1/2000
ICC: 1/200 - 1/1000
Flow Cytometry: 1/200 - 1/400
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Purified antibody in PBS with 0.05% sodium azide.
Concentration:
batch dependent
Storage Conditions:
BACE monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BACE1
Additional Names:
BACE Antibody: ASP2, BACE, HSPC104, KIAA1149, Beta-secretase 1, Aspartyl protease 2, ASP2
Ncbi Gene Id Number:
23621
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Multiple transcript variants encoding different isoforms have been described for this gene.
Background Reference 1:
J Neurochem. 2012 Jan;120 Suppl 1:62-70.
Background Reference 2:
Eur J Neurosci. 2010 Oct;32(7):1223-38.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-238

List Size:
0.1 mL

List Price:

$495.00

Datasheet
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