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Western blot analysis of BACE2 in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide with BACE2 antibody at 1 μg/mL.
Immunohistochemistry of BACE2 in rat heart tissue with BACE2 antibody at 2 μg/mL.
Western blot analysis of BACE2 in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide with BACE2 antibody at 1 μg/mL.
Trial Size

BACE2 Antibody

CATALOG NUMBER: 2249

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%)
Immunogen:
BACE2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human BACE2.
The immunogen is located within the last 50 amino acids of BACE2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
BACE2 antibody can be used for detection of BACE2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2:
Cat. No. 1461 - Rat Heart Tissue Lysate
Predicted Molecular Weight:
Predicted: 57 kDa
Observed: 57 kDa
Purification:
BACE2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
BACE2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BACE2
Additional Names:
BACE2 Antibody: ASP1, BAE2, DRAP, AEPLC, ALP56, ASP21, CDA13, CEAP1, UNQ418/PRO852, Beta-secretase 2, Aspartic-like protease 56 kDa, ASP1
Protein Accession Number:
AAF17078
Protein Gi Number:
6561812
Ncbi Gene Id Number:
25825
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BACE2 Antibody: Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by proteases. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE/Asp2 is a novel transmembrane aspartic protease and co-localizes with APP. A BACE homolog was recently cloned and designated BACE2, Asp1, DRAP (for Down region aspartic protease), and memapsin 1. BACE2 also cleaves APP at b-site and at a different site within Abeta. BACE2 locates on chromosome 21q22.3, the so-called ‘Down critical region', suggesting that BACE2 and Abeta may also contribute to the pathogenesis of Down syndrome.
Background Reference 1:
Vassar R, et al. β-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999;286:735-41
Background Reference 2:
Hussain I, et al. Identification of a novel aspartic protease (Asp 2) as β-secretase. Mol Cell Neurosci 1999;14:419-27
Background Reference 3:
Sinha S, et al. Purification and cloning of amyloid precursor protein β-secretase from human brain. Nature 1999;402:537-40
Background Reference 4:
Yan R, et al. Membrane-anchored aspartyl protease with Alzheimer's disease β-secretase activity. Nature 1999;402:533-7

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2249

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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