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Western blot analysis of lysed extracts from HT29 and HepG2 cells using BAD (Ab-136).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (Ab-136).
Western blot analysis of lysed extracts from HT29 and HepG2 cells using BAD (Ab-136).

BAD (Ab 136) Antibody

CATALOG NUMBER: 79-301

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
BAD (Ab-136) antibody was raised against a peptide sequence around aa.134~138 (S-R-S-A-P) derived from Mouse BAD.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of total BAD protein.
Predicted Molecular Weight:
23 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
BAD
Additional Names:
BBC2, BCL2L8, BBC6
Protein Accession Number:
NP_004313.1
Protein Gi Number:
10835069
Ncbi Gene Id Number:
572
Background:
The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Background Reference 1:
Wang XQ, et al. (2001) . J Biol Chem. 276 (48): 44504-44511. Lee YI, et al. (2001) . J Biol Chem. 276 (20): 16969-16977. Maiti D, et al. (2001) . J Biol Chem. 276 (1): 329-333.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-301

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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