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Western blot analysis of Bad in T24 cell lysates with Bad antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunohistochemical staining of rat thymus using Bad at 2 μg/mL.
Immunofluorescence of BAD in Rat Thymus cells with BAD antibody at 10 μg/mL.
Western blot analysis of Bad in T24 cell lysates with Bad antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Trial Size

BAD Antibody

CATALOG NUMBER: 3343

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Bad antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human Bad.
The immunogen is located within amino acids 90 - 140 of BAD.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Bad antibody can be used for detection of Bad by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1213 - T24 Cell Lysate
Positive Control 2:
Cat. No. 1471 - Rat Thymus Tissue Lysate
Purification:
BAD Antibody is Ion exchange chromatography purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BAD Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
BAD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BAD
Additional Names:
BAD Antibody: BBC2, BCL2L8, BBC6, Bcl2 antagonist of cell death, Bcl-2-binding component 6, BAD
Protein Accession Number:
Q92934
Protein Gi Number:
17371773
Ncbi Gene Id Number:
572
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BAD Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax. Bad activity is regulated by its phosphorylation; it is inactivated by kinases such as Akt and MAP kinase and thus promotes cell survival, whereas JNK-induced phosphorylation promotes the apoptotic role of Bad.
Background Reference 1:
Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
Background Reference 2:
Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
Background Reference 3:
Ottilie S, Diaz JL, Horne W, et al. Dimerization properties of human BAD. Identification of a BH-3 domain and analysis of its binding to mutant BCL-2 and BCL-XL proteins. J. Biol. Chem. 1997; 272:30866-72.
Background Reference 4:
Zhou XM, Liu Y, Payne G, et al. Growth factors inactivate the cell death promoter BAD by phosphorylation of its BH3 domain on Ser155. J. Biol. Chem. 2000; 275:25046-51.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3343

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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