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To a sandwich ELISA (assuming 100ul/well), a concentration of 5.0-6.0 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human BAFF when used with ProSci's biotinylated antigen affinity purified anti-human BAFF (XP-5105Bt) as the detection antibody at a concentration of approximately 0.25-0.50 ug/ml.
To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.
To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.
To a sandwich ELISA (assuming 100ul/well), a concentration of 5.0-6.0 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human BAFF when used with ProSci's biotinylated antigen affinity purified anti-human BAFF (XP-5105Bt) as the detection antibody at a concentration of approximately 0.25-0.50 ug/ml.

BAFF Antibody [2B]

CATALOG NUMBER: XP-5728-M

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
The monoclonal antibody was produced in BALB/c mice using recombinant human BAFF as the immunizing antigen.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 5.0-6.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human BAFF when used with our biotinylated antigen affinity purified anti-human BAFF (XP-5105Bt) as the detection antibody at a concentration of approximately 0.25-0.50 μg/mL.
Western Blot:
To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.
Purification:
This IgG1/K antibody was purified from ascites fluid on a Protein A chromatography column.
Clonality:
Monoclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
BAFF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
Ncbi Official Symbol:
TNFSF13B
Additional Names:
B cell Activating Factor , BAFF, BLYS, TALL1, TNFSF20, ZTNF4, UNQ401/PRO738, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, BLyS, DTL, CD257, THANK, TALL-1
Protein Accession Number:
Q9Y275
Protein Gi Number:
13124573
Ncbi Gene Id Number:
10673
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5728-M

List Size:
0.5 mg

List Price:

$376.00

Datasheet
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