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Western blot analysis of BAFF Receptor in human (H), mouse (M), and rat (R) spleen tissue lysates with BAFF Receptor antibody at 5 μg/mL.
Immunohistochemistry of BAFF-R in human spleen tissue with BAFF-R antibody at 5 μg/mL.
Immunofluorescence of BAFF-R in human spleen tissue with BAFF-R antibody at 20 μg/ml.<br><br>Red: BAFF Receptor Antibody (3097) <br> Blue: DAPI staining
Immunohistochemistry of BAFF Receptor in human tonsil tissue with BAFF Receptor antibody at 5 μg/mL.
Western blot analysis of BAFF Receptor in human (H), mouse (M), and rat (R) spleen tissue lysates with BAFF Receptor antibody at 5 μg/mL.
Trial Size

BAFF Receptor Antibody

CATALOG NUMBER: 3097

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
BAFF Receptor antibody was raised against a synthetic peptide corresponding to 15 amino acids near the carboxy terminus of human BAFF Receptor The peptide sequence is identical between human and mouse origin.
The immunogen is located within the last 50 amino acids of BAFF Receptor.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
BAFF Receptor antibody can be used for detection of BAFF Rexeptor by Western blot at 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2:
Cat. No. 1406 - Mouse Spleen Tissue Lysate
Positive Control 3:
Cat. No. 1466 - Rat Spleen Tissue Lysate
Positive Control 4:
Cat. No. 1315 - Human Tonsil Tissue Lysate
Positive Control 5:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
20 kDa
Purification:
BAFF Receptor Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BAFF Receptor Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
BAFF Receptor antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TNFRSF13C
Additional Names:
BAFF Receptor Antibody: BAFFR, CD268, CVID4, BAFF-R, BROMIX, prolixin, BAFFR, BR3, Tumor necrosis factor receptor superfamily member 13C, B-cell-activating factor receptor
Protein Accession Number:
AAK91826
Protein Gi Number:
15208475
Ncbi Gene Id Number:
115650
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BAFF Receptor Antibody: Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF, BLyS, TALL-1, THANK, and zTNF4. BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. Two receptors, TACI and BCMA, for BAFF were originally identified. A third receptor was identified recently and designated BAFF-R and BR3 for BLyS receptor 3. Unlike BCMA and TACI, which bind to BAFF and April, BAFF-R/BR3 is specific for BAFF and plays a predominant role in BAFF induced B cell development and survival. BAFF and its receptors are involved in B cell associated autoimmune diseases, and activate NF-κB and c-jun N-terminal kinase.
Background Reference 1:
Moore PA, Belvedere O, Orr A, et al. BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator. Science 1999;285:260-3
Background Reference 2:
Schneider P, MacKay F, Steiner V, et al. BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth. J Exp Med 1999;189:1747-56
Background Reference 3:
Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J Leukoc Biol 1999;65:680-3
Background Reference 4:
Mukhopadhyay A, Ni J, Zhai Y, Yu GL, Aggarwal BB. Identification and characterization of a novel cytokine, THANK, a TNF homologue that activates apoptosis, nuclear factor-κB, and c-Jun NH2-terminal kinase. J Biol Chem 1999 ;274:15978-81

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3097

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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