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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
BAG2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human ovarian carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

BAG2 Antibody

CATALOG NUMBER: 57-448

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This BAG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-176 amino acids from the C-terminal region of human BAG2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BAG2
Additional Names:
BAG family molecular chaperone regulator 2, BAG-2, Bcl-2-associated athanogene 2, BAG2
Protein Accession Number:
O95816
Ncbi Gene Id Number:
9532
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BAG proteins compete with Hip for binding to theHsc70/Hsp70 ATPase domain and promote substrate release. All theBAG proteins have an approximately 45-amino acid BAG domain nearthe C terminus but differ markedly in their N-terminal regions. Thepredicted BAG2 protein contains 211 amino acids. The BAG domains ofBAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPasedomain in vitro and in mammalian cells. All 3 proteins bind withhigh affinity to the ATPase domain of Hsc70 and inhibit itschaperone activity in a Hip-repressible manner. [provided byRefSeq].
Background Reference 1:
Arndt, V., et al. Mol. Biol. Cell 16(12):5891-5900(2005)
Background Reference 2:
Dai, Q., et al. J. Biol. Chem. 280(46):38673-38681(2005)
Background Reference 3:
Ueda, K., et al. J. Biol. Chem. 279(40):41815-41821(2004)
Background Reference 4:
Ueda, K., et al. J. Biol. Chem. 279(40):41815-41821(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-448

List Size:
400 ul

List Price:

$495.00

Datasheet
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