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46-977 (1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-977 (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-977 (1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

BAG4 Antibody

CATALOG NUMBER: 46-977

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-GNATSDHPNNQDQSS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 60kDa band observed in cell lines HEK293 and HeLa lysates (calculated MW of 49.6kDa according to NP_004865.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Takada et al, Mol Cell Biol. 2003 Jun;23(11):4026-33.; PMID: 12748303). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1210 - 293 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BAG4
Additional Names:
BAG4, BCL2-associated athanogene 4, BAG-4, SODD, BAG-family molecular chaperone regulator-4, silencer of death domains
Protein Accession Number:
NP_004865.1
Protein Gi Number:
6631075
Ncbi Gene Id Number:
9530
Background Reference 1:
Annunziata CM, Kleinberg L, Davidson B, Berner A, Gius D, Tchabo N, Steinberg SM, Kohn EC. BAG-4/SODD and associated antiapoptotic proteins are linked to aggressiveness of epithelial ovarian cancer. Clin Cancer Res. 2007 Nov 15;13(22 Pt 1):6585-92

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-977

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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