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HEK293 lysate (10ug protein in RIPA buffer) over expressing Human BAIAP2 with DYKDDDDK tag probed with 45-017 (1.0 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with 45-017 (1mg/ml) in Lane B. Pri
45-017 (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
HEK293 lysate (10ug protein in RIPA buffer) over expressing Human BAIAP2 with DYKDDDDK tag probed with 45-017 (1.0 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with 45-017 (1mg/ml) in Lane B. Pri

BAIAP2 Antibody

CATALOG NUMBER: 45-017

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-SMSSADVEVARF
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:In transfected HEK293 transiently expressing full-length Human BAIAP2 (myc and DYKDDDDK tagged), a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
Specificity:
This antibody will recognise only one of three reported isoforms (NP_006331.1).
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BAIAP2
Additional Names:
BAIAP2, BAP2, IRSP53, BAI1-associated protein 2, FLAF3, brain-specific angiogenesis inhibitor 1-associated protein 2, fas ligand-associated factor 3, insulin receptor substrate p53, insulin receptor substrate p53/p58
Protein Accession Number:
NP_006331.1
Protein Gi Number:
5453565
Ncbi Gene Id Number:
10458
Background Reference 1:
Oda K, Shiratsuchi T, Nishimori H, Inazawa J, Yoshikawa H, Taketani Y, Nakamura Y, Tokino T. Identification of BAIAP2 (BAI-associated protein 2), a novel human homologue of hamster IRSp53, whose SH3 domain interacts with the cytoplasmic domain of BAI1. Cytogenet Cell Genet 1999;84(1-2):75-82

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-017

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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