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Western blot analysis of Bak in L1210 cell lysates with Bak antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of Bak in L1210 cells with Bak antibody at 2 μg/mL.
Western blot analysis of Bak in L1210 cell lysates with Bak antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

Bak Antibody

CATALOG NUMBER: 3347

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Bak antibody was raised against a peptide corresponding to 13 amino acids near the amino-terminus of human Bak.
The immunogen is located within amino acids 30 - 80 of Bak.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
Bak antibody can be used for detection of Bak by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1284 - L1210 Cell Lysate
Positive Control 2:
Cat. No. 17-204 - L1210 Cell Slide
Purification:
Bak Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Bak Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Bak antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BAK1
Additional Names:
Bak Antibody: BAK, CDN1, BCL2L7, BAK-LIKE, BAK, Apoptosis regulator BAK, Bcl2-L-7
Protein Accession Number:
Q16611
Protein Gi Number:
2493274
Ncbi Gene Id Number:
578
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bak Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bak, a pro-apoptotic Bcl-2 family member, is an oligomeric protein that localizes to the mitochondria. It is thought to share significant functional homology with Bax, another pro-apoptotic Bcl-2 family member, as disruption of bak or bax has little effect on cell death, but mice lacking both genes display multiple developmental defects and cells lacking bak and bax show decreased apoptotic capability.
Background Reference 1:
Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
Background Reference 2:
Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
Background Reference 3:
Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
Background Reference 4:
Kiefer M, Brauer MJ, Powers VC, et al. Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak. Nature 1995; 374:736-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3347

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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