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Western blot analysis of BANP in mouse kidney tissue lysate with BANP antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of BANP in human kidney tissue with BANP antibody at 10 μg/mL.
Immunofluorescence of BANP in human kidney tissue with BANP antibody at 20 μg/mL.
Western blot analysis of BANP in mouse kidney tissue lysate with BANP antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

BANP Antibody

CATALOG NUMBER: 5977

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
BANP antibody was raised against a 14 amino acid synthetic peptide near the center of human BANP.
The immunogen is located within amino acids 300 - 350 of BANP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
BANP antibody can be used for detection of BANP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
BANP Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BANP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
BANP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BANP
Additional Names:
BANP Antibody: BEND1, SMAR1, SMARBP1, BEND1, Protein BANP, BEN domain-containing protein 1
Protein Accession Number:
NP_001167014
Protein Gi Number:
291084803
Ncbi Gene Id Number:
54971
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BANP Antibody: BANP was initially identified as a binding protein to BTG3 in a yeast two-hybrid screen. BANP acts as a tumor suppressor by stabilizing p53 expression and leading to cell cycle arrest. p53 in turn binds to upstream elements of the BANP promoter, thereby forming a feedback loop. BANP is down-regulated in advanced stages of human breast cancer, and its overexpression in breast cancer cell lines inhibits their ability to metastasize by modulating TGF-beta signaling. Furthermore, BANP can modulate NF-κB transactivation and can inhibit tumorigenesis by regulating NF-κB target genes. Recent experiments have shown that BANP can also repress HIV-1 LTR mediated transcription by tethering the LTR matrix attachment region to nuclear matrix.
Background Reference 1:
Birot A, Duret L, Bartholin L, et al. Identification and molecular analysis of BANP. Gene2000; 253:189-96.
Background Reference 2:
Kaul R, Mukherjee S, Ahmed F, et al. Direct interaction with and activation of p53 by SMAR1 retards cell-cycle progression at G2/M phase and delays tumor growth in mice. Int. J. Cancer2003; 103:606-15.
Background Reference 3:
Singh K, Mogare D, Giridharagopalan RO, et al. P%3 target gene SMAR1 is dysregulated in breast cancer: its role in cancer cell migration and invasion. PLoS One2007; 2:e660.
Background Reference 4:
Signh K, Sinha S, Malonia SK, et al. Tumor suppressor SMAR1 repsses IkBa expression and inhibits p65 transactivation through matrix attachment regions. J. Biol. Chem.2009; 284:1267-78.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5977

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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