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Western blot analysis of Bap29 in human heart tissue lysate with Bap29 antibody at (A) 0.5, (B) 1 and (C) 2μg/mL.
Immunohistochemistry of BAP29 in human heart tissue with BAP29 antibody at 10 μg/mL.
Immunofluorescence of BAP29 in Human Heart cells with BAP29 antibody at 20 μg/mL.
Western blot analysis of Bap29 in human heart tissue lysate with Bap29 antibody at (A) 0.5, (B) 1 and (C) 2μg/mL.
Trial Size

BAP29 Antibody

CATALOG NUMBER: 3667

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (88%)
Immunogen:
BAP29 antibody was raised against a 17 amino acid synthetic peptide from near the center of human Bap29 Bap29 antibody is specific for isoform a of Bap29.
The immunogen is located within amino acids 130 - 180 of BAP29.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Bap29 antibody can be used for the detection of Bap29 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2:
Cat. No. 10-501 - Human Heart Tissue Slide
Purification:
BAP29 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BAP29 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
BAP29 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BCAP29
Additional Names:
BAP29 Antibody: BAP29, BAP29, B-cell receptor-associated protein 29, BCR-associated protein 29
Protein Accession Number:
NP_001008405
Protein Gi Number:
56549093
Ncbi Gene Id Number:
55973
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BAP29 Antibody: Bap29 and the related protein Bap31 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Both Bap29 and Bap31 interact with membrane-bound immunoglobulins (mIgs), such as IgM and IgD, which with Ig-alpha/Ig-beta heterodimers form B cell antigen receptors. Binding of the Bap29/Bap31 heterodimer correlates with the ER retention of non-Ig-alpha/Ig-beta bound mIg complexes, suggesting that Bap29 and Bap31 may act as chaperones transmembrane regions of various proteins. Bap29 possesses multiple isoforms.
Background Reference 1:
Kim KM, Adachi T, Nielsen PJ, et al. Two new proteins preferentially associated with membrane immunoglobulin D. EMBO J.1994; 13:3793-800.
Background Reference 2:
Ng F, Nguyen M, Kwan T, et al. p28 BAP31, a Bcl-2/Bcl-XL- and procaspase-8-associated protein the endoplasmic reticulum. J. Cell Biol.1997; 139:327-38.
Background Reference 3:
Schamel WW, Kuppig S, Becker B, et al. A high-molecular-weight complex of membrane proteins BAP29/BAP31 is involved in the retention of membrane-bound IgD in the endoplasmic reticulum. Proc. Natl. Acad. Sci. USA2003; 100:9861-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3667

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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